Table 3.
List of polymorphic SSR markers (31) and their details
| SSR | Primer sequences | Source | Band size (bp) | NA | Ta (°C) | h |
|---|---|---|---|---|---|---|
| Rh79 |
F: TTCTTCTTGCTCGCCATTTTGATT R:GAACGTCCACCACCACCCACTCTG |
Yan et al. (2005) | 135, 142, 149, 160, 174 | 5 | 50 | 0.76 |
| RhAB9 |
F:GTCAATTTGTGCATAAGCTC R:GTGAGAACAGATGAGAAATG |
Yan et al. (2005) | 101, 116, 124 | 3 | 50 | 0.6 |
| Rh48 |
F:GATAGTTTCTCTGTACCCCACCTA R:TTGACCAGCTGCAACAAAATTAGA |
Yan et al. (2005) | 99, 107, 127, 136, 144 | 5 | 50 | 0.39 |
| Rh80 |
F:CATGCCAAACGAAATGAGTTA R:TTATCTAAAGGGCTGCTGTAAGTT |
Yan et al. (2005) | 134, 148 | 2 | 50 | 0.47 |
| Rh96 |
F:GCCGATGGATGCCCTGCTC R: AGATTCCCTGCGACATTCACATTC |
Yan et al. (2005) | 276, 282, 306 | 3 | 50 | 0.44 |
| Rh50 |
F: TGATGAAATCATCCGAGTGTCAG R: TCACTTTCATTGGAATGCCAGAAT |
Yan et al. (2005) | 240, 303, 340 | 3 | 50 | 0.49 |
| Rh58 |
F: ACAATTTAGTGCGGATAGAACAAC R: GGAAAGCCCGAAAGCGTAAGC |
Yan et al. (2005) | 238, 258, 274, 286, 291, 300 | 6 | 50 | 0.78 |
| RhABT12 |
F: CAAGTTTGTCTCCTTGGACC R: CATAGATGATTATCCTAGAGCC |
Yan et al. (2005) | 156,174,200,225 | 4 | 50 | 0.44 |
| Rh65 |
F: AGTACGCCGACGCAGATCCAGTGA R: ACGGCGTTGTAGGTCGTCATTCTC |
Yan et al. (2005) | 128,140,160 | 3 | 50 | 0.54 |
| Rh78 |
F: AAAGAAACGCGAAATCTATGATGC R: TCTGGATGGGATTTAAAAGACAGG |
Yan et al. (2005) | 216,296,336 | 3 | 50 | 0.52 |
| Rh77 |
F: CAACTGAAAGGAACAAATGGATGT R: GGAATGGCTTGTAAATTTGTGATT |
Yan et al. (2005) | 246,262,280,309 | 4 | 50 | 0.69 |
| Rh93 |
F: GCTTTGCTGCATGGTTAGGTTG R: TTCTTTTTGTCGTTCTGGGATGTG |
Yan et al. (2005) | 244,275 | 2 | 50 | 0.45 |
| RhAB38 |
F: GAGGTGGTCGATTCCATGTC R: TTACCGTTCTACCTAAGTGACTAAC |
Yan et al. (2005) | 120,139,173 | 3 | 50 | 0.55 |
| Rh60 |
F: TCTCTTTTCACGGCCACCACT R: TGAATCCAAGGCCGTATAGTTAGA |
Yan et al. (2005) | 244, 272, 300 | 3 | 50 | 0.51 |
| Rh85 |
F: ACTTTTGGGCGTTCATCGCATTACAC R:GGCTATATGGGCTCAAGTCTAGACAA |
Yan et al. (2005) | 203, 221 | 2 | 50 | 0.48 |
| Rh98 |
F: GGCCTCTAGAGTTTGGGATAGCAG R: ACGACGTCAATAACTCCATCAGTC |
Yan et al. (2005) | 160, 221 | 2 | 50 | 0.28 |
| Rh72 |
F: CCAAAAGACGCAACCCTACCATAA R: TCAAAACGCATGATGCTTCCACTG |
Yan et al. (2005) | 254, 276, 282, 310 | 4 | 50 | 0.58 |
| Rh73 |
F: GGTTAGACGGGTGGAAGAAG R: ACTGCCGATAGAAGTATTTCATCA |
Yan et al. (2005) | 150, 175, 205 | 3 | 55 | 0.62 |
| RhAB28 |
F: GCAGATGTTATTCATGTTAA R: CCAAGTATTTTAGTTTCTTC |
Yan et al. (2005) | 164, 180, 190 | 3 | 55 | 0.64 |
| ABR II/Rpu4 |
F: CTTGTTCAAAGGGTTCTCTG R: CACCTAAATGATGCTTTTCC |
Jowkar et al. (2009) | 274, 312 | 2 | 50 | 0.49 |
| ABRII/Rpu7 |
F: GAAACCCAGTTATTGATGCCTGGA R: CCAGATTATTGATGCCTGAT |
Jowkar et al. (2009) | 213, 220 | 2 | 50 | 0.49 |
| ABRII/Rpu10 |
F: AAGATTGGTGTTGGGTGTTA R: CTCGTTCTGGTTCTGTCTTC |
Jowkar et al. (2009) | 115, 118, 122 | 3 | 50 | 0.15 |
| ABR II/RPU11 |
F: AGTTGGACCTGTTTTCTTCA R: AGCACGACGACGAGTTTC |
Jowkar et al. (2009) | 205, 236, 248, 275 | 4 | 57 | 0.69 |
| ABRII/RPU12 |
F: GAAGAAGAACGACTGAGAGC R: GAGCAGAGAATTTCCATTTG |
Jowkar et al. (2009) | 103,109,117,131 | 4 | 50 | 0.5 |
| ABRII/RPU14 |
F: AGCACTTACAGGCTCATCAT R: CCTCCAAGTCAAGTTCTACG |
Jowkar et al. (2009) | 206, 216, 222, 230 | 4 | 50 | 0.52 |
| ABRII/RPU32 |
F: TAGAGCTATTTTCGATTCGG R: GGGTGACACAGAGAGAGAGA |
Jowkar et al. (2009) | 238, 265 | 2 | 50 | 0.08 |
| ABRII/RPU33 |
F: TCTCTCTCTGTGTCACCCTC R: CGTCTCCCTCTTCTTCTTCT |
Jowkar et al. (2009) | 150, 180 | 2 | 50 | 0.29 |
| ABRII/Rpu36 |
F: TAGTTGAGAGCTCGGAGAAG R: GAAGTTACAAGACGAAACCG |
Jowkar et al. (2009) | 235, 255 | 2 | 50 | 0.29 |
| RA013a |
F: GAGGGAAAGAGATACACAAA R: GTAAGACCTTGCGTGTTCATA |
Kimura et al. (2006) | 145, 155 | 2 | 55 | 0.37 |
| RA023b |
F: CATCCTCGGTGTTGCGTTGA R: TGTCTCCAGCAACCTTTTTTTCCC |
Kimura et al. (2006) | 174, 194 | 2 | 50 | 0.49 |
| RA043a |
F: GCAACGTACTTCAATTTCCAC R: CAAGCTCAGAACTGAGACAC |
Kimura et al. (2006) | 123, 136, 149, 179 | 4 | 50 | 0.63 |
Primer sequence motifs (F: forward and R: reverse); Ta, Annealing temperatures; band sizes of each amplified marker; NA, number of the amplified fragments generated by SSR primers; h, Nei’s expected heterozygosity values for markers