Table 3.
Ranking of the reference gene and their expression stability values calculated by Normfinder
Ranking order | Species and cultivars of Forsythia | Organs of Forsythia plants | Developmental stages of Forsythia flower buds | Forsythia flower plants | Developmental stages of Forsythia fruits | Leaves of Forsythia plants treated with ABA | Leaves of Forsythia plants treated with PEG | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene | Stability | Gene | Stability | Gene | Stability | Gene | Stability | Gene | Stability | Gene | Stability | Gene | Stability | |
1 | TIP | 0.174 | GAPDH | 0.664 | TIP | 0.137 | SDH | 0.104 | ACT | 0.132 | ACT | 0.324 | G6PD | 0.036 |
2 | UKN1 | 0.290 | SDH | 1.039 | ACT | 0.220 | UKN1 | 0.139 | TIP | 0.140 | TIP | 0.491 | UKN1 | 0.132 |
3 | G6PD | 0.323 | G6PD | 1.109 | SDH | 0.220 | ACT | 0.212 | UKN1 | 0.143 | PTB | 0.533 | SDH | 0.182 |
4 | ACT | 0.410 | PTB | 1.182 | G6PD | 0.256 | PTB | 0.267 | G6PD | 0.148 | UKN1 | 0.537 | TIP | 0.546 |
5 | TUB | 0.497 | UKN1 | 1.357 | UKN1 | 0.430 | TIP | 0.388 | ABCT | 0.263 | G6PD | 0.553 | ACT | 0.593 |
6 | CYP | 0.566 | F-Box | 1.488 | CYP | 0.561 | TUB | 0.524 | PTB | 0.341 | SDH | 0.591 | MTP | 0.647 |
7 | SDH | 0.626 | TIP | 1.803 | GAPDH | 0.818 | MTP | 0.575 | MTP | 0.397 | TUB | 0.665 | TUB | 0.700 |
8 | PTB | 0.724 | ABCT | 1.822 | PTB | 0.926 | F-Box | 0.641 | SDH | 0.432 | MTP | 0.912 | CYP | 0.792 |
9 | F-Box | 0.996 | ACT | 2.139 | MTP | 0.980 | ABCT | 0.901 | F-Box | 0.663 | CYP | 1.071 | PTB | 0.941 |
10 | MTP | 1.017 | MTP | 2.214 | TUB | 1.111 | G6PD | 0.912 | TUB | 1.071 | F-Box | 1.212 | F-Box | 1.096 |
11 | GAPDH | 1.732 | CYP | 2.252 | F-Box | 1.321 | CYP | 0.932 | CYP | 1.147 | GAPDH | 1.355 | GAPDH | 1.738 |
12 | ABCT | 2.337 | TUB | 2.503 | ABCT | 1.417 | GAPDH | 1.283 | GAPDH | 1.909 | ABCT | 2.268 | ABCT | 2.108 |
Stability values was calculated by Normfinder and the smaller the stability value, the more suitable it is as reference gene