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. 2019 Nov 30;9(3):485–506. doi: 10.1016/j.jcmgh.2019.11.012

Figure 3.

Figure 3

Flagellin-specific CD4+T cells are enriched proportionally in IBD patients. (A) Gating strategy and representative flow cytometry data for cohort 3 for all antigens tested (n = 20 each for healthy controls, CD patients, and UC patients). The percentage of CD25+OX40+ cells within CD4+ T cells after stimulation of whole blood for 44 hours with (B) SEB or Pediacel, or (C) FliC or Fla2 antigens. (D) Anti-FliC and anti-Fla2 for subjects with detectable (>0.02% of CD4+ T cells) Pediacel-specific CD4+ T cells (n = 19 healthy, n = 17 CD, n = 18 UC), the proportions of FliC- and Fla2-specific T cells were analyzed as a proportion of Pediacel-specific CD4+ T cells within each individual (Kruskal–Wallis test). PE, phycoerythrin.