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. 2019 Nov 26;9(3):507–526. doi: 10.1016/j.jcmgh.2019.11.008

Figure 10.

Figure 10

Modulation of PGE2-induced changes in mucus layer thickness by ion channel inhibition. (A) Dark-field side-view images of a Colon Chip with epithelial cells and mucus layer before (no PEG2) and after PGE2 treatment for 4 hours without (PGE2 control) or with CFTRinh-172 (50 μmol/L), XE-991 dihydrochloride (20 μmol/L), bumetanide (100 μmol/L), or a combination of all 3 ion channel inhibitors. Images are representative of 2 independent experiments. Scale bar: 200 μm. (B) Quantification of changes in mucus layer height based on the dark-field images shown in panel A and (C) the total mucin production measured using an Alcian blue assay induced by PGE2 in the absence (PGE2 control) or presence of CFTRinh-172 (50 μmol/L), XE-991 dihydrochloride (20 μmol/L), bumetanide (100 μmol/L), or a combination of all 3 ion channel inhibitors (data are presented relative to control chips treated with PGE2 alone; n = 2–6; 2 independent experiments compiled). **P < .01 vs control. All data represent means ± SEM.