Figure 2.

Epithelium development in the Colon Chip after 3, 7, and 14 days of culture after monolayer formation. (A) Phase-contrast microscopic images of colonic epithelial cells cultured on the Colon Chip. Scale bars: 200 μm. (B) Differential interference contrast images of the colonic epithelium (viewed from above). Scale bars: 100 μm. (C) Three-dimensional confocal microscopic reconstruction of z-stack images of the epithelial cells based on F-actin staining. Images are representative of 2 independent experiments. Scale bars: 100 μm. (D) Differential interference contrast images of the full length of the channel of the Colon Chip. Images are representative of 2 independent experiments. Scale bars: 1000 μm.