Table 3.
Comparison of RhCE phenotyping, adsorption-elution test, and RHD genotyping
| RhCE phenotyping | Adsorption-elution test | RHD genotyping | |
|---|---|---|---|
| Cost1 TAT1 Hands-on time1 |
USD 34.5 (USD 5.8/sample) 30 min 30 min (5 min/sample) |
USD 120.0 (USD 20.0/sample) 4.5 h 3.5 h (35 min/sample) |
USD 106.5 (USD 17.8/sample) 2.5 h 1.5 h (15 min/sample) |
| Advantages | Rapid, inexpensive, and no special instruments required | No special instruments required, phenotypic characterization of novel alleles | Relatively rapid and an accurate (reference method) |
| Disadvantages | Only used to exclude D-negative samples with RHD null alleles | Technically demanding, laborious, and time-consuming | Usually unable to detect all DEL alleles High initial costs for instruments |
1
Cost, TAT, and hands-on time were estimated, assuming that 6 samples were tested simultaneously.