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. 2020 Feb 19;7(1):ENEURO.0397-19.2019. doi: 10.1523/ENEURO.0397-19.2019

Figure 1.

Figure 1.

Analysis of DRG from two different spinal levels (lumbar: L4-5; sacral: L6-S1) taken from five male and seven female adult C57Bl/6 mice. A, Principal component analysis (N = 23 168; all detected genes) indicates spinal region as the major source of variance in the data. B, Volcano plot illustrates differential expression between lumbar and sacral DRG. Light gray points are genes not differentially expressed, dark gray points are differentially expressed at adjusted p < 0.05; examples of specific differentially expressed genes are highlighted in red. A negative value for the FC indicates an upregulation in sacral DRG, whereas a positive FC indicates upregulation in lumbar DRG. Full dataset are provided in Extended Data Figure 1-1. C, Heatmap with hierarchical cluster for all differentially expressed genes (N = 466; adjusted p < 0.05) for adult lumbar and sacral samples (N = 12). Both samples and rows are clustered using Pearson correlation. Heat color reflects row-wise z score, and samples are colored according to spinal level and sex. Ranked gene lists for heat map are provided in Extended Data Figure 1-2.