Figure 2.

Gene set enrichment analysis of DRG neurons taken from five male and seven female adult C57Bl/6 mice, comparing expression at lumbar (L4-5) and sacral (L6-S1) levels. Seven of 10 gene sets (grouped according to shared function; see Extended Data Fig. 2-1) were enriched (adjusted p < 0.05): LGICs, TFs, GPCRs, NTFRs, Ca, and K channels, and TRP channels. A, B, Five gene classes showed upregulation in sacral DRG (blue) and two showed upregulation in lumbar DRG (red). C, D, Genes differentially expressed between lumbar and sacral spinal levels from the LGIC and GPCR classes. Blue indicates upregulation in sacral DRG and red upregulation in lumbar DRG. Full list of differentially genes identified by this analysis is provided in Extended Data Figure 2-2, and complete set of heatmaps is provided in Extended Data Figure 2-3.