Figure 5.

Gene set enrichment analysis of Trpv1-sorted DRG neurons taken from five male and five female adult mice, comparing expression at lumbar (L4-5) and sacral (L6-S1) levels. Nine of 10 gene sets (grouped according to shared function) were enriched (adjusted p < 0.05): LGICs, TFs, GPCRs, NTFRs, K, Ca, and Cl channels, and TRP channels. A, B, All nine gene classes showed upregulation in sacral DRG (blue). C–F, Genes differentially expressed between lumbar and sacral spinal levels from the LGIC, GPCR, K, and Ca channel classes. Blue indicates upregulation in sacral DRG and red upregulation in lumbar DRG. Full list of genes identified by this analysis is provided in Extended Data Figure 2-2, and a complete set of heatmaps is provided in Extended Data Figure 5-1.