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. 2020 Feb 19;7(1):ENEURO.0397-19.2019. doi: 10.1523/ENEURO.0397-19.2019

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Copyright © 2020 Smith-Anttila et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Analysis of DRG from two different spinal levels (lumbar: L4-5; sacral: L6-S1) taken from five male and five female E18.5 mouse embryos. A, Volcano plot illustrating genes differentially expressed between lumbar and sacral DRG neurons. Light gray points are genes not differentially expressed, dark gray points are differentially expressed at adjusted p < 0.05 (N = 466); examples of specific differentially expressed genes are highlighted in red. A negative value for the FC indicates an upregulation in sacral DRG, whereas a positive FC indicates upregulation in lumbar DRG. Full dataset provided in Extended Data Figure 6-1. B, Proportional Venn diagram showing the number of genes differentially expressed between spinal levels (pooled male and female data), in the total E18.5 and total adult DRG population. A total of 466 genes were differentially expressed between lumbar and sacral levels in the total population of adult DRG neurons (adjusted p < 0.05), 111 of which were also detected as differentially expressed between lumbar and sacral levels in E18.5 DRG neurons. An additional 1109 genes were detected as differentially expressed between lumbar and sacral levels only in the E18.5 DRG population. Gene lists summarized in Venn diagram are provided in Extended Data Figure 6-2. C, Heatmap with hierarchical cluster for all differentially expressed genes (N = 1220; adjusted p < 0.05) for E18.5 lumbar and sacral samples (N = 10). Both samples and rows are clustered using Pearson correlation. Heat color reflects row-wise z score, and samples are colored according to spinal level and sex. Ranked gene lists for heat map (clusters 1 and 2) are provided in Extended Data Figure 1-2.