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. 2019 Aug 22;118(4):873–884. doi: 10.1016/j.bpj.2019.08.017

Figure 6.

Figure 6

Red edge excitation shift (REES) of NBD-labeled loop residues of KvAP-VSD. (A) The effect of changing excitation wavelength on the wavelength of maximum emission for G114-NBD in micelles (open circles) and POPC/POPG membranes (solid circles) is shown. The solid lines are the fit to Eq. 4. The magnitude of REES (B) and the relative area (C) calculated by fitting the REES data using Eq. 4 for all the labeled loop residues in micelles (white bars) and membranes (shaded bars) are shown. All other conditions are as in Fig. 2. See Materials and Methods for other details.