Figure 4.

T39 ASO-induced resistance to diet-induced obesity is IFNAR1 dependent. Littermate WT and Ifnar1−/− mice were treated with either the control or T39 ASO and placed on an HFSC diet for a total of 5 weeks. A) Body weight gain measured weekly after starting on HFSC diet. B) gWAT weight after 5 weeks HFSC diet. (C–E) Relative fold change of mRNA expression in SVF, adipocytes, or total gWAT measured by qRT-qPCR and normalized to actin and cyclophilin expression. Data represent mean ± SD (A,B) or SEM (C–E), n = 8–11 mice/group, *p < 0.05, **p < 0.01, ***p < 0.001.