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. 2020 Jan 30;34:146–156. doi: 10.1016/j.molmet.2020.01.010

Figure 6.

Figure 6

ASOs targeting human mRNAs can induce a type I IFN response in cultured THP1 cells. THP1 cells were differentiated into macrophages with 100 nM PMA for 3 days then transfected with ASO for 2 days and collected immediately in trizol for RNA extraction (A,C) or given poly I:C at the indicated doses for 12 h before collection (B,D). Data are the relative fold change of mRNA expression normalized to actin and cyclophilin expression treated with A,B) control or T39 ASO 923, 915, 139, or 990 or C,D) control, Ldlr ASO 326, 328, 306, or 311. B,D) The relative fold change of IFNβ expression after 12 h poly I:C dose response. n = 3 experiments/group (each experiment with 3–4 replicates), data plotted as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.