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. 2019 Sep 16;118(4):885–897. doi: 10.1016/j.bpj.2019.09.009

Figure 1.

Figure 1

AdipoRon suppresses Wnt-driven signaling. (A) Quantification of luciferase reporter activity from mouse fibroblasts (Leading Light 3T3) cells stably expressing an LEF luciferase reporter vestor. Cells were pretreated with 0.1% DMSO or indicated doses of AdipoRon for 12 h before stimulation, with 100 ng/mL of recombinant murine Wnt3a for 12 h in the presence of DMSO or AdipoRon (n = 3 wells per treatment). (B) Quantification of luciferase reporter activity. Mouse fibroblasts were pretreated with 0.1% DMSO or 10 μM AdipoRon for 12 h before stimulation with Wnt pathway activators (100 ng/mL Wnt3a, 500 ng/mL R-Spondin-1, 50 mM LiCl, or 2.5 μM CHIR99021) for 12 h in the presence of DMSO or AdipoRon (n = 3 wells per treatment). (C) Murine colonic organoid metabolic activity assessed by CellTiter-Blue Cell Viability Assay (n = 3 wells per treatment per time point). Unless otherwise indicated, data are mean ± SE; statistical significance between treatments (∗p < 0.01) was determined using (A) one-way ANOVA with Dunnett’s multiple comparisons test or (B and C) an unpaired t-test. To see this figure in color, go online.