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. 2019 Sep 16;118(4):885–897. doi: 10.1016/j.bpj.2019.09.009

Figure 5.

Figure 5

AdipoRon reroutes PM and extracellular cholesterol into lysosomes. (A) YAMC cells were treated with 0.1% DMSO or 10 μM AdipoRon for 48 h before staining lysosomes with 50 nM LysoTracker Red DND-99 for 30 min before fixing with 4% PFA for 15 min. After fixation, free cholesterol was stained with 50 μg/mL Filipin III for 45 min at room temperature in the dark. Zoom scale bar, 2 μm. DKOB8 cells were treated with 0.1% DMSO or 10 μM AdipoRon for 24 h in either (B) 5% FBS or (C) 5% cholesterol-depleted FBS then stained and fixed as described above. To see this figure in color, go online.