Figure 3.

PL-induced Ca²⁺ entry from the extracellular space in bEND5 cells. (A) To monitor exclusively intracellular Ca²⁺ mobilization, bEND5 cells were stimulated with 20% PL in the absence of external Ca²⁺ (0Ca²⁺). When Ca²⁺ has been added back to the medium, there was [Ca²⁺]_i increase. (B) Mean ± s.e.m. of the Ca²⁺ response at time zero and to 20% PL recorded after PL addition (first peak) and at the peak after Ca²⁺ supplement in extracellular space (second peak). Data are means ± s.e.m. of [Ca²⁺]_i measured by confocal imaging of 40 cells from 3 experiments Different asterisks on bars indicate statistical differences by one-way ANOVA according to Tukey’s test (p < 0.001).