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Berberine induced apoptosis in a dose and time dependent manner. Annexin-V and PI staining after 144 h of the treatment with berberine at 0.2, 0.5 and 1 µM. Western blot results of three independent experiments showing apoptosis related proteins expression upon berberine’s treatment at 120 h and 144 h. (a) MDA-MB-468, (b) BT-20, and (c) HCC70. ±SD of three independent experiments, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to DMSO. Berb—Berberine.
