Figure 2.

Future experiments for transposon insertional mutagenesis screens. (A,B) To identify drivers, induce tumor formation with insertional mutagenesis and implant tumors orthotopically into syngeneic recipients. (A) The primary tumor is expanded and removed before endpoint, allowing the metastatic lesions to expand and be harvested for driver analysis. (B) Implanted tumors are treated with a targeted inhibitor or left untreated and their drivers compared. (C) Mice undergoing mutagenesis are fed a normal or high fat diet. At endpoint all tumors are harvested and their drivers compared.