Table 2.
Published Sleeping Beauty and piggyBac Cancer Screens in Mice.
| Tumor Type | Transposase | Transposon | Cre | Sensitizing Mutations | Refs |
|---|---|---|---|---|---|
| Sarcomas | |||||
| Fibrosarcoma | CGS-SB10 | T2/Onc | - | p19arf | [20] |
| Osteosarcoma | R26-lsl-SB11 | T2/Onc | Osx-Cre | Trp53 | [48] |
| Peripheral nerve sheath tumor | R26-lsl-SB11 | T2/Onc, T2/Onc15 | Cnp-Cre, Dhh-Cre |
Trp53, EGFR
Nf1 |
[29,49] |
| Histiocytic sarcoma | R26-lsl-SB11 | T2/Onc, T2/Onc2 | Lyz2-Cre | - | [50] |
| Carcinomas | |||||
| Skin | K5-SB11 | T2/Onc2 | - | Hras | [25] |
| Mammary | K5-SB11, R26-lsl-SB11 | T2/Onc2, T2/Onc3 | K5-Cre, Wap-Cre | Trp53, β-catenin, Cdh1, FGFR, Pten | [51,52,53,54,55] |
| Pancreatic | R26-lsl-SB11, R26-lsl-SB13, R26-lsl-PB | T2/Onc, T2/Onc2, T2Onc3, ATP1 | Pdx1-Cre | Kras | [24,56,57] |
| Gastric adenoma | R26-lsl-SB11 | T2/Onc3 | β-actin-Cre | Smad4 | [58] |
| Intestinal tract | R26-lsl-SB11 | T2/Onc, T2/Onc2 | Vil-CreERT2, Vil-Cre, Ah-Cre | Apc, Kras, Smad4, Trp53, Tgfbr2 | [59,60,61,62,63] |
| Liver | R26-lsl-SB11 | T2/Onc, T2/Onc2, T2/Onc3 | Alb-Cre | HBsAg, Trp53, Myc, Steatosis, Pten, Sav1, Met | [27,64,65,66,67,68,69] |
| Lung | R26-lsl-SB11 | T2/Onc | Spc-Cre | Trp53, p19arf, Pten | [70] |
| Prostate | CGS-SB10, R26-SB11, R26-lsl-SB11 | T2/Onc, T2/Onc3 | PB-Cre | p19arf, Pten | [71,72] |
| Thyroid | R26-lsl-SB11 | T2/Onc2 | Tpo-Cre | Hras | [73] |
| Melanoma | R26-lsl-SB11, R26-lsl-SB13, Act-PBase |
T2/Onc, T2/Onc2, T2/Onc3
Luc-PB[mut]7 |
Tyr-Cre-ERT2 | Braf | [74,75,76,77] |
| Hematopoietic | |||||
| T cell leukemia | R26-lsl-SB11, R26-SB11 | T2/Onc2 | Vav-iCre, Lck-Cre, CD4-Cre | - | [21,78] |
| T cell lymphoma | R26-SB11, R26-lsl-PB | T2/Onc, ATP2 | CD4-Cre | Trp53, ITK-SYK, Pdc1 | [79,80] |
| B cell leukemia | R26-lsl-SB11, Etv6-RUNX1-HSB5 | T2/Onc | Cd79a-Cre | Stat5b, Etv6-RUNX1 fusion | [81,82] |
| B cell lymphoma | R26-lsl-SB11, Etv6-RUNX1-HSB5, R26-PB | T2/Onc, T2/Onc2, T2/Onc15, ITP1, ITP2 | Aid-Cre, CD19-Cre, Cnp-Cre | Eμ-TCL1, Pax5, Etv6-RUNX1 fusion, Trp53, Pten, Blm | [23,83,84,85,86] |
| Acute myeloid leukemia | R26-lsl-SB11 | T2/Onc2, GrOnc | β-actin-Cre, Vav-Cre, Mx1-Cre | Trp53, Jak2, Npm1c, BCR-ABL | [87,88,89,90] |
| Mixture of T cell and B cell lymphoma, myeloid leukemia | R26-SB11 | T2/Onc | - | Rassf1a, Cadm1 | [91,92] |
| Erythroleukemia | R26-lsl-SB11 | T2/Onc2 | Mx1-Cre | Cyclin E | [93] |
| Myeloid and lymphoid malignancies, thymus, spleen | R26-lsl-SB11 | T2/Onc2 | Vec-Cre | - | [94] |
| Brain tumors | |||||
| Medulloblastoma/CNS-ET * | R26-lsl-SB11, R26-SB11, Math1-SB11 | T2/Onc, T2/Onc2, T2/Onc3 | β-actin-Cre, Nestin-Cre | Ptch1, Trp53, Pten | [28,95,96,97,98,99] |
| Glioma | R26-lsl-SB11, R26-SB11 | T2/Onc, T2/Onc2, T2/Onc3, T2OncATG | Nestin-Cre | Trp53, p19Arf, Blm, Csf1 | [26,100,101] |
| Multiple tumor types | |||||
| Skin, brain, airway, liver, leukemia, lymphoma, intestine | R26-SB11 | T2/Onc3 | - | Rag2 | [102] |
| Leukemia, medulloblastoma, glioma | CGS-SB10, R26-SB11 | T2/Onc | - | p19Arf | [22] |
| Skin, liver, lung, brain, lymphoma, sarcoma, mammary, colon, etc. | R26-SB11 | T2/Onc3 | - | - | [23] |
| T cell and B cell leukemia, lymphoma, skin, sarcoma, intestinal tract, lung, liver, etc. | R26-PB | ATP1, ATP2, ATP3 | - | - | [103] |
| Prostate, mammary and skin carcinomas | R26-SB11 | ITP2m | - | Pten, Blm | [104] |
| Sarcoma, carcinoma, leukemia, resistance to MDM2 inhibition | R26-PB | ATP2 | - | p19Arf | [105] |
| Liver, lung carcinoma, skin carcinoma, lymphoma | R26-PB | ATP1 | - | - | [106] |
* CNS-ET—Embryonal tumor of the central nervous system.