Table 2.
Effects of melatonin on assisted reproductive technology under in vitro conditions. COC: cumulus oocyte complex; IVM: in vitro maturation; M II: metaphase II; ROS: reactive oxygen species; MT1, MT2: melatonin membrane receptors; BMP: bone morphogenic protein; PTX3: pentraxin-3; HAS2: hyaluronan synthase 2; EGFR: epidermal growth factor receptors; GSH: glutathione; ATP: adenosine triphosphate; GDF: growth differentiation factor; SOD: superoxide dismutase; GPX: glutathione peroxidase; Bcl-2: B-cell lymphoma-2; GSX: reduced glutathione; OCT4: octamer-binding transcription factor 4; H2AX: histone H2 family member X; H3K4me: methylation of lysine4 on histone H3; H4K27me: methylation of lysine27 on histone H3; CAT: catalase; HSP: heat shock protein; MTNR1A: melatonin receptor 1A; MT: melatonin receptor; 4P-PDOT: 4-phenyl-2-propionamidotetralin; BimEL: Bcl-2 interacting mediator of cell death extra-long; ERK: extracellular signal-regulated kinase; H3K9: histone H3 lysine 9.
| Animal | Design | Melatonin Treatment | Result | Year | Author/Reference |
|---|---|---|---|---|---|
| mouse | vitro COCs | 10−6 M | Cumulus expansion, M-Ⅱ ↑ ROS, Acetyla on level of H4k12 ↓ |
2017 | Keshavarzi Somayeh [51] |
| mouse | vitro, IVM, implantation | 10−7 M | blastocyst rate, hatching blastocyst rate and blastocyst cell number ↑ pregnancy rate and birth rate↑, (ROS) production and cellular apoptosis ↓ |
2017 | Tian Xiuzhi [69] |
| sheep | vitro, IVM | 10−7 M | rates of nuclear maturation, cumulus cells expansion, cleavage, and blastocyst ↑ MT1 and MT2 were expressed in oocytes, cumulus cells, and granulosa cells BMP15, PTX3, HAS2, EGFR ↑, cAMP level ↓, cGMP ↑ |
2017 | Tian Xiuzhi [65] |
| bovine | vitro, IVM | 10−9 M | ROS↓, GSH↑,mitochondrial normal distribution increase ATP level upregulated ATPase 6, BMP-15, GDF-9, SOD-1, Gpx-4, and Bcl-2 downregulated apoptotic gene expression of caspase-3. |
2017 | Yang Minghui [68] |
| porcine | vitro IVM COCs | 10−7, 10−6, 10−5 M | oocyte quality, embryo development ↑ ROS generation, apoptosis, and DNA damage ↓, GSX, OCT4, H2AX |
2018 | Lin Tao [52] |
| bovine | vitro, IVM | 10−9 M | G1 blastocyst ↑, cell number ↑, apoptotic cell ↓ glutathione content, mitochondrial membrane potential ↑ antioxidant gene (SOD2) heat shock protein (HSPB1) ↑ |
2018 | Marques TC [53] |
| porcine | vitro prolonged culture | 10−3 M | blastocyst rate↑ methylation at H3K4me2 and H3K27me2 ↓ imprinted gene NNAT ↓ |
2018 | Nie Junyu [71] |
| bovine | vitro, IVM | 10−9 M | blastocyst, total cell number ↑, apoptotic cell ↓ ROS ↓, GSH ↑ caspase-3 ↓, BCL-2, XIAP, CAT, HSP70 ↑ |
2018 | Pang Yunwei [66] |
| Goat | vitro, IVM | 10−9 M, 10−12 M | M-II stage, blastocyst ↑, GSH ↑, MTNR1A in cumulus cell and oocytes DNA methyltransferases (DNMTs) global DNA methylation ↓ |
2018 | Saeedabadi Saghar [63] |
| mouse | vitro, IVM | 10 μM | fertilization rate ↑ hyaluronan synthase-2 (HAS2) and Progesterone receptor (PGR) ↑ |
2018 | Ezzati Maryam [70] |
| porcine | vitro COCs | 10−9 M | blastocyst, cell number, cumulus expansion ↑, apoptosis ↓ MT2 was expressed in both oocytes and cumulus cells M effects were abolished when either luzindole or 4P-PDOT (MT antagonist) |
2018 | Lee Sanghoon [62] |
| porcine | vitro COCs | 10−9 M | pro-apoptotic protein BimEL, ERK-mediated phosphorylation M only promoted the ubiquitination of phosphorylated BimEL M action was independent of its receptor and its antioxidant properties |
2018 | Wang Yingzheng [67] |
| bovine | vitro cloned embryo | 10−9 M | cloned embryo development ↑ oxidative stress, apoptosis, mitochondria, chromosome alignment epigenetic modifications, H3K9 acetylation ↑, H3K9 methylation ↓ |
2019 | An Quanli [56] |