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. 2020 Feb 6;21(3):1068. doi: 10.3390/ijms21031068

Figure 2.

Figure 2

Identification and subcellular localization of StABI5 transgenic potatoes. (A) Identification of StABI5 transgenic potato lines by leaf PCR. The amplified PCR product was 1547 bp when using primers P35S F and StABI5 R. De: Desiree. (B) Western blot analysis of StABI5 protein expression in StABI5 transgenic potato lines. (C) The transcription level of StABI5 in StABI5 transgenic potato lines obtained by qRT-PCR. StACTIN58 was used as an internal control. (D) Subcellular localization of StABI5 in tobacco (Nicotiana tabacum). Tobacco transiently expressed Agrobacterium strain containing the PStABI5::StABI5-GFP vector, and the localization of GFP was observed by laser confocal microscopy. Bars, 50 μm.