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. 2020 Feb 3;21(3):994. doi: 10.3390/ijms21030994

Figure 2.

Figure 2

(A) AAV-mediated gene delivery to retinal organoids. Schematic of the protocol for generating and infecting human retinal organoids with representative images from each step. rAAV infection is performed at day 44 of differentiation. The efficiency of gene delivery illustrated on day 70 corresponds to one-single infection using AAV2-7m8-GFP at a dose of 5 × 1010 vg per organoid. (B) Expression of AAV receptor KIAA0319L in mature mouse retina, human retina and hiPSC-derived organoids. Representative retinal cross-section of wild-type mouse (B) or human retina (C) stained with antibodies against the KIAA0319L receptor. (D) Expression of AAVR KIAA0319L in Day 44 old hiPSC-derived organoids. (E) Zoom into the dotted square depicted in D. (F) Expression of AAVR KIAA0319L in day 70 old hiPSC-derived organoids. (G). Zoom into the dotted square depicted in F. Scale bars: A and D = 50 μm; B–C; E–F = 20 μm.