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. 2020 Feb 3;21(3):994. doi: 10.3390/ijms21030994

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AAV mediated reporter gene expression in relation to the distribution of AAV receptors in hiPSC-derived retinal organoids. (A) Confocal image of a D70 retinal organoid infected with AAV2-7m8-CAG-GFP, GFP signal was amplified using chicken polyclonal anti-GFP antibody. (B) RT-PCR showing expression of co-receptor genes (Laminin receptor1 (RPSA) and FGFR1) in the mature human retina, D40 and D70 retinal organoids. (C–F) Confocal images showing localization of Syndecan 3 and Laminin receptor 1 in 3D retinal organoids at different stages of differentiation. Scale bars: A, 50 μm; C–H, 20 μm.