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. 2020 Feb 3;21(3):994. doi: 10.3390/ijms21030994

Figure 6.

Figure 6

AAV mediated gene delivery to hiPSC derived RPE. (A–D) Epifluorescence images of the expression of the RPE structural marker ZO1 (red), the reporter protein GFP (green), and the nucleus marker DAPI (blue) in hiPSC-derived RPE cultures infected with different AAVs. Scale bar: 50 µm (A) AAV2-CAG-GFP, (B) AAV2-7m8-CAG-GFP, (C) AAV8-CAG-GFP, (D) AAV9-CAG-GFP. (E) Percentage (%) of GFP positive cells in hiPSC-derived RPE cultures infected with different capsids quantified using ImageJ analysis. N = 3. Values are mean ± SEM. For statistical significance Mann–Whitney Student’s test was used and ***p < 0.05 was considered significant. n.s. was used to declare non significant.