Table 2.
Purification of pantocinwh-1 produced by Pantoea dispersa W18.
| Method | Vol(ml) | Total Activity (AU) 1 | Yield (%) | Total Protein (mg) 2 | Purification (fold) |
|---|---|---|---|---|---|
| Supernatant | 2000 | 1.60 × 106 | 100 | 16,520 | 1 |
| SP-Sepharose | 350 | 5 × 105 | 80 | 3537 | 75 |
| Octyl-Sepharose | 120 | 5 × 105 | 80 | 623 | 212 |
| RP-HPLC | 4 | 2.3 × 105 | 23 | 1.52 | 963 |
1 Antibacterial activity (expressed in arbitrary units (AU)) was assayed by the spot-on-lawn method using M. smegmatis mc2 155 as an indicator strain. 2 The protein concentration (in mg/mL) was estimated with a NanoDrop ND-1000 (A280).