Table 1.
LC-MS identification of wheat gliadin spots in 2-DE. The protein name, accession number, mass, pI, protein score, protein match, protein coverage, peptide sequence and mass error (ppm) were presented.
| Spot | G1 | G2 | G3 |
|---|---|---|---|
| Predominant protein | Gamma gliadin | Gamma gliadin | Gamma gliadin |
| Accession number | F2XAR5 | Q30DX7 | K7WVC4 |
| MW | 37,564 | 34,502 | 33,067 |
| pI | 8.51 | 8.57 | 8.88 |
| Protein score | 94 | 1762 | 2831 |
| Protein match | 2 | 91 (54) | 62 (52) |
| Protein coverage | - | 25.5 | 15.8 |
| Sequence | R.APFASIVAGIGGQ. (ppm: 5.18) R.SLVLQTLPSMCSVYVPPECSIMR.A (ppm: −44.56) |
R.TTTSVPFGVGTGVGAY. (ppm: 8.86) R.ILPTMCSVNVPLYR.T (ppm: 6.64) R.ILPTMCSVNVPLYR.T (ppm: 6.85) K.VFLQQQCSPVAMPQR.L (ppm: 9.19) R.SQMLQQSSCHVMQQQCCQQLPQIPQQSR.Y (ppm: 8.80) |
R.SDCQVMQQQCCQQLAQIPR.Q (ppm: 6.96) R.SDCQVMQQQCCQQLAQIPR.Q (ppm: 0.35) R.QPQQPFYQQPQQTFPQPQQAFPHQPK.Q (ppm: 5.81) |
The UniProt database was utilized to the spectral datasets searching.