Figure 1.

Egm inhibits CD4⁺ T cell activation and cytokine responses.

Notes: (A) OT-II whole splenocytes were incubated with whole OVA protein at various concentrations for 72 hrs in the presence of DMSO (vehicle) or 10 µM Egm in DMSO. (B) Egm dose response of OT-II whole splenocytes incubated with OVA_323-339 peptide (50 µg/mL) for 72 hrs. IFN-γ concentration was measured via ELISA. (C) Representative flow cytometric analysis of OT-II whole splenocyte cultures incubated with OVA_323-339 peptide in the presence of DMSO or 10 µM Egm in DMSO for 72 hrs. T cell activation was evaluated by analysis of CD44^hiCD62L^lo T cell populations (plots gated from TCRβ⁺CD4⁺ T cells). (D) Quantification of CD44^hiCD62L^lo T cell populations from 3 biological replicates. (E) Quantification of CD69 expression in the presence of Egm or DMSO from 3 biological replicates. The significance of the data was evaluated via ordinary One-way ANOVA with multiple comparison test. (*P<0.05).

Abbreviations: OVA, ovalbumin; DMSO, dimethyl sulfoxide; No Tx, no treatment.