Table 1.
Primers Used in This Study
| Target Gene | Sequence (5ʹ – 3ʹ) | Amplicon Size (bp) | Annealing Temperature |
|---|---|---|---|
| toxA | F: 5ʹGGTAACCAGCTCAGCCACAT 3ʹ | 352 | 48°C |
| R: 5ʹTGATGTCCAGGTCATGCTTC 3’ | |||
| plcN | F: 5ʹ GTTATCGCAACCAGCCCTAC 3ʹ | 466 | 48°C |
| R: 5ʹAGGTCGAACACCTGGAACAC | |||
| plcH | F: 5ʹGAAGCCATGGGCTACTTCAA 3ʹ | 307 | 48°C |
| R: 5ʹAGAGTGACGAGGAGCGGTAG 3’ | |||
| algD | F:5ʹ ATGCGAATCAGCATCTTTGGT 3ʹ | 1311 | 48°C |
| R:5ʹ CTACCAGCAGATGCCCTCGGC 3ʹ | |||
| lasB | F:5ʹCCAGCCCGCTGACCCACAAGCTGTA 3ʹ | 650 | 46°C |
| R: 5ʹCATTCCTTCCTGGAGTGCYRGCCG 3’ | |||
| aprA | F:5ʹ CCTGATCKGGCCGATAACTGCAAT 3ʹ | 1580 | 46°C |
| R:5ʹ GGAAGACASCTATCAATTCGAACAG 3’ | |||
| exoU | F: 5ʹ GGCACATATCTCCGGTTCCTTC 3ʹ | 761 | 55°C |
| R: 5ʹ TCAACTCAGCTGCCAACCATGC 3’ | |||
| exoS | F: 5ʹATGGCGTGTTCCGAGTCA 3ʹ | 1587 | 55°C |
| R: 5ʹ AGGTGTCGGTTCGTGACGTCT 3’ | |||
| ERIC primers | ERIC-1R, 5VCACTTAGGGGTCCTCGAATGTA-3V | ||
| ERIC-2, 5V-AAGTAAGTGACTGGGGTGAGCG-3V |
Abbreviations: toxA, exotoxin A; plcH, hemolytic phospholipase C; plcN, non-hemolytic phospholipase C; algD, GDP-mannose dehydrogenase enzyme for alginate; lasB, elastase; aprA, alkaline protease; exoU, exoenzyme U; exoS, exoenzyme S; ERIC, Enterobacterial Repetitive Intergenic Consensus-PCR.