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. 2020 Jan 31;21(3):932. doi: 10.3390/ijms21030932

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Number of Pax7+ cells in TA section of mdx and mdxθ^−/− mice at 6 and 12 weeks, and 6 and 12 months of age. The number of Pax7⁺ cells was calculated by counting the number of PAX7⁺ cells per field of view in immune-stained sections. The results are the means ± SD (n = 3/ genotype/ age (* p < 0.05, ** p < 0.01, two-tailed Student’s t-test). (B) Myogenin mRNA levels in WT (n = 2), PKCθ^−/− (n = 3), mdx (n = 5) and mdxθ^−/− (n = 4) TA muscle from 12 months old mice. The values were normalized vs. GAPDH mRNA level of expression. Statistical significance was determined by a one-way ANOVA using a Tukey’s post-test (* p < 0.05, means ± SD).