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. 2020 Feb 9;21(3):1143. doi: 10.3390/ijms21031143

Figure 2.

Figure 2

Myeloperoxidase (MPO)−/− or pharmacological MPO inhibition by ABAH prevents aberrant brain capillary sphingolipid homeostasis in response to peripheral LPS. (A,B) Cer and SM content of microcapillaries (pooled from three brains per sample) of PBS or LPS (8.3 µg/g body weight) injected MPO−/− mice were analyzed by LC-ESI-MS/MS. The values were normalized to the internal standard and protein amount. Data are shown as mean (n = 4) + SD. (C,D) Wt mice were injected with PBS or LPS (8.3 µg/g body weight) ± ABAH (40 µg/g body weight; injected 2 h before and 5 h after PBS or LPS injection). Cer and SM content of microcapillaries (pooled from three brains per sample) was analyzed by LC-ESI-MS/MS. Data are shown as mean (n = 7) + SD. (E) Wt mice received a single i.p. injection of PBS or LPS (8.3 µg/g body weight) ± ABAH (40 µg/g body weight; injected twice: 2 h before and 5 h after PBS or LPS injection) and Evans Blue (3% in PBS; 4 µL/g body weight). Twelve hours post treatment, animals were anaesthetized with pentobarbital (150 mg/kg body weight) and transcardially perfused with 25 mL PBS. Brains were removed, frozen in liquid nitrogen, and homogenized. Evans Blue was quantitated spectrophotometrically using an external Evans Blue calibration curve. Results represent mean values (PBS: n = 14, PBS+LPS: n = 13, PBS+ABAH: n = 13, PBS+ABAH+LPS: n = 6) ± SD. Significance was calculated by ANOVA, followed by Bonferroni correction. *, p ≤ 0.05; ***, p ≤ 0.001.