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. 2020 Feb 9;21(3):1143. doi: 10.3390/ijms21031143

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Short term metabolic cage readouts 24 h after first LPS injection. Wt and MPO^−/− mice were housed at room temperature in metabolic cages with free access to chow diet and water. Mice were injected daily with LPS (i.p. 0.83 µg/g body weight) for 5 d. (A) Total (blue box represents the 24 h period after the first LPS injection) and (B) mean respiratory exchange ratio (RER), (C) carbohydrate oxidation, (D) lipid oxidation, (E) daily food intake, (F) water consumption, (G) locomotor activity, and (H) energy expenditure were measured for 24 h after the first LPS injection. Data represent means (n = 6) + SD. Significance was calculated by student’s unpaired t-test, followed by Welch’s correction in case of unequal variances. *, p ≤ 0.05; **, p < 0.01.