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. 2020 Feb 19;13:1519–1531. doi: 10.2147/OTT.S236158

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© 2020 Sun et al.

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Up-regulated lncRNA ENST00000512916 promotes AM-1 cell cycle progression and cell-cycle-related protein expression. Flow cytometry assay results showing the significant increased G1/G2 phase in AM-1 cells transfected with si-ENST00000512916 (A), and the significant increased S phase in AM-1cells transfected with ENST00000512916 overexpression (B). Real-time qPCR results showed the mRNA expression levels of cell-cycle-related proteins including CyclinD1, CDK2, CDK4 and CDK6 were significantly inhibited in AM-1 cells transfected with si-ENST00000512916 (C) or were significantly promoted in AM-1 cells transfected with ENST00000512916 overexpression (D). (E) Western blotting analysis showed that the expression levels of CDK2, CDK4 and CyclinD1 were significantly inhibited in AM-1 cells transfected with si-ENST00000512916. (F) Western blotting analysis showed that the expression levels of CDK2, CDK4 and CDK6 were significantly elevated in AM-1 cells transfected with ENST00000512916 overexpression. *p-value<0.05; **p-value<0.01; ***p-value<0.001; ****p-value<0.0001 and ns indicates no statistical significance.