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. 2020 Feb 19;13:1569–1581. doi: 10.2147/OTT.S237307

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© 2020 Sun et al.

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Identification and validation of circ_0058124 in TC cells.

Notes: (A) The relative expression levels of circ_0058124 and mFN1 in TPC-1 and HTH83 cells were analyzed by q-PCR after normalized with random primers and oligo (dT)₁₈ primers. (B) The expression levels of circ_0058124, U6 and GAPDH in the nuclear and cytoplasmic of TPC-1 and HTH83 cells were detected by q-PCR after nuclear-cytoplasmic separation. (C) The relative expression levels of circ_0058124 and mFN1 in TPC-1 and HTH83 cells were assessed by q-PCR after treatment with RNase R. (D) The relative expression levels of circ_0058124 and mFN1 in TPC-1 and HTH83 cells were analyzed by q-PCR after treatment with ActD at the indicated time points. *P < 0.05.