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. 2020 Feb 19;13:1569–1581. doi: 10.2147/OTT.S237307

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© 2020 Sun et al.

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Circ_0058124 could sponge miR-940.

Notes: (A) The sequences of circ_0058124 containing the miR-940 binding sites or mutant binding sites were shown. (B) Dual-luciferase reporter assay was used to detect the interaction between miR-940 and circ_0058124 in TPC-1 and HTH83 cells. (C) The enrichment of circ_0058124 and miR-940 in anti-Ago2 or anti-IgG was determined by the RIP assay. (D) RNA pull-down assay was used to assess the enrichment of circ_0058124 in Bio-miR-940 or Bio-NC. (E) Q-PCR was performed to measure the expression of miR-940 in TC tissues (TC) and adjacent normal tissues (Normal). (F) The expression of miR-940 in TC cells (TPC-1 and HTH83) and NTHY-ORI3.1 cells was determined by q-PCR. (G) Circ_0058124 expression was determined by q-PCR to evaluate the transfection efficiency of circ_0058124 overexpression plasmid in TPC-1 and HTH83 cells. (H) The expression of miR-940 was detected by q-PCR in TPC-1 and HTH83 cells to assess the effects of si-circ_0058124 and circ_0058124 overexpression plasmid on miR-940 expression. *P < 0.05.