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. 2020 Feb 19;13:1569–1581. doi: 10.2147/OTT.S237307

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© 2020 Sun et al.

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Effects of miR-940 on the progression of TC cells.

Notes: TPC-1 and HTH83 cells were co-transfected with miR-940 mimic and circ_0058124 overexpression plasmid. (A) Q-PCR was used to detect the expression of miR-940 to evaluate the transfection efficiency of miR-940 mimic and circ_0058124 overexpression plasmid. (B) The number of colonies in TPC-1 and HTH83 cells was measured by colony formation assay. (C and D) Transwell assay was used to determine the number of migrated and invaded TPC-1 and HTH83 cells. (E and F) Seahorse XF Extracellular Flux Analyzer was used to assess the OCR of basal respiration and maximum respiration in TPC-1 and HTH83 cells. *P < 0.05.