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. 2020 Feb 19;13:1569–1581. doi: 10.2147/OTT.S237307

Figure 7.

Figure 7

Effects of MAPK1 on the progression of TC cells.

Notes: TPC-1 and HTH83 cells were co-transfected with si-MAPK1 and anti-miR-940. (A) WB analysis was used to assess the protein level of MAPK1 to evaluate the transfection efficiency of si-MAPK1 and anti-miR-940. (B) Colony formation assay was performed to detect the number of colonies in TPC-1 and HTH83 cells. (C and D) Transwell assay was used to measure the number of migrated and invaded TPC-1 and HTH83 cells. (E and F) The OCR of basal respiration and maximum respiration in TPC-1 and HTH83 cells was determined by Seahorse XF Extracellular Flux Analyzer. *P < 0.05.