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. 2020 Jan 22;21(3):722. doi: 10.3390/ijms21030722

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of the activation of the tyrosine kinase receptors MET and Recepteur d’Origine Nantais (RON) in MKN45 gastric multicellular tumor spheroids (MCTS) overexpressing α2,3-sialylation after crizotinib treatment. Gastric MCTS were generated using the 3D Petri Dish^® technology (MICROTISSUES^®) for 5 days and treated with different concentrations of crizotinib for 48 h. (A) Western blot analysis of MET and RON receptor tyrosine kinases and their activated forms, pMET and pRON. GAPDH was used as a loading control. (B) Results of the automated image analysis of the immunofluorescence staining of gastric MCTS (Supplementary Material Figure S1) subjected to different concentrations of crizotinb. No significant differences were found (Student’s t-test, p-value > 0.05).