Figure 5.

MT dynamics is essential for myelination. (A) Experimental timeline of myelinating co-cultures of OPCs and dorsal root ganglion (DRG) explants (left). Illustrations of myelinating oligodendrocytes are shown on right. DRGs were cultured and maintained for 14 days, and purified OPCs were added to the DRG explant culture at DIV 0. Co-cultures were treated with MTAs (2 nM) at DIV 12 and fixed at DIV 14. Myelination proceeds through three stages, contacting, extending, and wrapping. (B) Representative images of co-cultures stained with anti-neurofilament (NF) and anti-MBP antibodies. Scale bar, 25 µm. (C) Quantification of myelination. Myelinating oligodendrocytes were categorized as depicted in A. Graph shows mean ± SEM. n = 4. * p < 0.05, ** p < 0.01, and *** p < 0.001, Mann–Whitney test.