Figure 2.

Adhesion and survival of human urothelial cells (HUCs) on natural spider silk fibers (A) Live (green) and dead (red) staining of cells cultured on spider silk. Insert: Magnified view of the indicated (white box) area. (B,C) Confocal images of actin filaments and filopodia development of HUC cells cultured on spider silk meshes after one day of culture showing DAPI (blue) and phalloidin (red)-staining. Attachment sites of cells on fiber meshes are shown with white arrows (B), and cells bridging the gap between two fibers are shown with a yellow arrow (C). (D) Scanning electron microscopy (SEM) image showing cells cultured on spider silk meshes after one day of culture. Consecutive magnifications are shown in sequence. The black arrow points towards the filopodia of cells spread on the fiber surface. Adopted and modified with permission [45]. Copyright 2015, Public Library of Science (PLOS).