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Formation of heterodimer by oligonucleotides r(G4C2)4 and r(G2C4)4. (a) 1H-NMR spectra of r(G4C2)4, r(G2C4)4 and their equimolar mixture (r(G4C2)4 + r(G2C4)4) at pH 6.0 and 25 °C. (b) 1H-NMR spectra (r(G4C2)4 + r(G2C4)4) at 7 °C before and after annealing of the sample. Δ represents NMR spectrum after annealing. All spectra were acquired at 0.2 mM oligonucleotide concentration per sense and antisense strand. c) Schematic presentation of heterodimer adopted by r(G2C4)4 and r(G4C2)4.
