Figure 5.

Effect of pH, presence of K⁺ ions and PEG on heterodimer. (a) ¹H-NMR spectra of annealed equimolar mixture of r(G₄C₂)₄ and r(G₂C₄)₄. Δ represents NMR spectrum after second annealing. All spectra were acquired on an 800 MHz NMR spectrometer at 25 °C and 0.2 mM oligonucleotide concentration per strand. (b) ¹H-NMR spectra of annealed equimolar mixture of r(G₄C₂)₄ and r(G₂C₄)₄ in the presence of 100 mM K⁺ and PEG at different pHs acquired at 25 °C and 0.2 mM oligonucleotide concentration per sense and antisense strand. Numbers under signals in the bottom spectrum represent integral values. c) Proposed model of heterodimer in the presence of 10% w/v PEG at pH 4.5.