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. 2020 Jan 21;21(3):693. doi: 10.3390/ijms21030693

Figure 2.

Figure 2

SHPRH localization in the nucleus is dependent on APIM. (A) Overexpressed RALIK-YFP (mutAPIM in SHPRH), RFLIK-CFP (APIM in SHPRH), and HcRed-PCNA. (B) Overview of subcellular localization of GFP-SHPRH and GFP-SHPRH F1632A, and (C) Quantification of nuclear localization of GFP-SHPRH (n(number of foci)= 484) and GFP-SHPRH F1632A (n = 123), and GFP-SHPRH after treatment with an APIM peptide (n = 247) or mutAPIM-peptide (n = 319), average of three biological replica, normalized to untreated control, two-sided Student’s t-test, * p < 0.05, *** p < 0.001. (D) Overexpressed GFP-SHPRH and HcRed-PCNA and (E) GFP-SHPRH F1632A and HcRed-PCNA. All images are from live cells. Scale bar = 5 μm. (F) Quantification of PCNA level pulled down by anti-GFP from YFP-HLTF, YFP-HLTF F960A, GFP-SHPRH, and GFP-SHPRH F1632A transfected cells after weak cross-linking and MMS treatment. Level of PCNA is given as % of total GFP-protein pulled down. Two independent biological replicas are shown.