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. 2020 Jan 21;21(3):688. doi: 10.3390/ijms21030688

Figure 3.

Figure 3

Production of region 3 of perlecan. (A) Schematic of the domain organization of perlecan. Amino acid numbering for the proteins and the domain constructs are shown. Different domains are colored separately: laminin IV type A-like domain (green), laminin Epidermal Growth Factor (EGF)-like domain (red) and unassigned sequence regions (black). Numbering (1–5) shows different regions of perlecan [30]. Region 3 contains 3 laminin IV type A-like domains and 11 laminin EGF-like domains. The laminin IV type A-like domains split the laminin EGF-like domains 1, 5 and 9 into two parts (where “f” stands for first part, “s” for second part, “t” stands for truncated). (B) Representative coomassie stained SDS-PAGE gels of reduced and non-reduced samples of purified region 3 constructs of perlecan. Only single bands are visible in the non-reduced samples suggesting that the proteins are in a single redox state.