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. 2019 Oct 28;217(1):e20190682. doi: 10.1084/jem.20190682

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© 2019 Yang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure S5. — Silencing of mosGILT in WT A. gambiae does not influence Vg expression and P. berghei infection. (a) mosGILT knockdown efficiency in the injected X1 females was validated by RT-qPCR of SG cells 4 d after dsRNA injection. Data are representative of more than three experiments (each dot represents five mosquitoes, mean ± SD, unpaired t test, **, P < 0.01). (b) Transcript levels of AgVg in the fat body of dsluc or dsmosGILT injected females 24 h after feeding on P. berghei–infected mice. Data represent duplicate experiments from two experiments (each dot represents five mosquitoes, mean ± SD, unpaired t test, ns, not significant). (c and d) P. berghei oocyst number (c) and infection prevalence (d) in the midgut 8 dpi. Each dot in panel c represents the oocyst number in one mosquito midgut (data pooled from two independent experiments, X1 dsluc n = 34, X1 dsmosGILT n = 31, Mann–Whitney test, ns, not significant). The horizontal lines (red) indicate the medians. Each dot in panel d represents one independent experiment (mean ± SD, Fisher’s exact test, ns, not significant).