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. 2019 Oct 21;217(1):e20190832. doi: 10.1084/jem.20190832

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© 2019 Long et al.

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Figure 3. — Nsd2 is required for Tfh differentiation in a cell-intrinsic manner. (A and B) Nsd2^CD4WT and Nsd2^CD4KO OT II T cells were adoptively transferred into congenically distinct receipt mice, immunized with OVA (A) or SRBC-OVA (B), and analyzed at days 3 and 5. Data are representative of four independent experiments. (C) Nsd2^CD4WT and NSD2^CD4KO OT II T cells were adoptively transferred into CD28^−/− mice, immunized with NP-OVA, and analyzed for GC B cells at day 7. Data are representative of three independent experiments. (D) CD4^CreERT2+RosaYFP⁺Nsd2^+/+ and CD4^CreERT2+RosaYFP⁺Nsd2^fl/fl mice on OT II TCR transgenic background were treated with tamoxifen for 5 d consecutively, and OT II T cells from these mice were adoptively transferred into recipient mice, which were subjected to OVA immunization and analyzed for Tfh at day 3. n = 10. Data were pooled from three experiments. In all bar graphs, bars represent means, and dots represent individual mice. Error bars show means ± SD. Statistical analysis was done with Student’s t test. **, P < 0.01; ***, P < 0.001.