Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 25;217(1):e20190972. doi: 10.1084/jem.20190972

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Hosokawa et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Bcl11b-binding molecules in pro-T and ILC2 cells. (A) Total extracts from Myc-Flag-Bcl11b–expressing ILC2/b6 cells were subjected to two-step affinity purification followed by SDS-PAGE and silver staining. All of the visible bands were subjected to mass spectrometry analysis. (B) Representative Bcl11b-binding molecules in pro-T cells, identified previously (Hosokawa et al., 2018a), and ILC2/b6 are shown with Mascot scores in Scid.adh.2c2 and ILC2/b6 cells. The full list of Bcl11b-binding molecules is shown in Table S2. (C) Nuclear lysates from Scid.adh.2c2 and ILC2/b6 cells were subjected to immunoblotting (I.B.) with the indicated antibodies. (D) Total extracts from Mock- or Myc-Flag-Bcl11b–transduced Scid.adh.2c2 and ILC2/b6 cells were subjected to immunoprecipitation (IP) with anti-Flag mAb followed by immunoblotting with the indicated antibodies. Data in A, C, and D are representative of two independent experiments.