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. 2019 Oct 23;217(1):e20182325. doi: 10.1084/jem.20182325

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© 2019 Liu et al.

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Figure 5. — The gastric microbiota is dispensable for disease development in GEC C1galt1^−/− mice. (A) Abx treatment regimen. (B) FISH using the EUB338 probe on gastric sections of 6-mo-old mice. Dashed line, interface between lumen (lu) and mucosa (m). Scale bars, 20 µm. (C) Representative qPCR for the 16S ribosomal RNA gene from genomic DNA extracted from stomach contents (mean ± SEM; n = 5 mice/group). (D) H&E of antrum sections. Graphs on right; gastritis activity index. Scale bars, 50 µm. (E) Western blot analysis of stomach lysates. (F) Densitometry of Casp1 p10 subunit in Western blot (mean ± SEM). (G) ELISA for IL1β and IL18 in serum. Results are representative of three or four mice per group (mean ± SEM). *, P < 0.05.