Table 3.
IC50 and selective index values of MEBV and AERC.
| Crude Extracts | Parasites | IC50 (µg/mL) a | EC50 (µg/mL) b | Selective Indices c | ||||
|---|---|---|---|---|---|---|---|---|
| MDBK | NIH/3T3 | HFF | MDBK | NIH/3T3 | HFF | |||
| MEBV | B. bovis | 0.84 ± 0.2 | 695.7 ± 24.9 | 931 ± 44.9 | >1500 | 828.2 | 1108.3 | >1785.7 |
| B. bigemina | 0.81 ± 0.3 | 858.9 | 1149.3 | >1851.9 | ||||
| B. divergens | 4.1 ± 0.9 | 169.7 | 227.1 | >365.9 | ||||
| B. caballi | 0.35 ± 0.1 | 1987.7 | 2660 | >4285.7 | ||||
| T. equi | 0.68 ± 0.1 | 1023.1 | 1369.1 | >2205.9 | ||||
| AERC | B. bovis | 85.7 ± 3.1 | 737.7 ± 17.4 | >1500 | >1500 | 8.6 | >17.5 | >17.5 |
| B. bigemina | 55.7 ± 2.7 | 13.2 | >26.9 | >26.9 | ||||
| B. divergens | 90 ± 3.7 | 8.2 | >16.7 | >16.7 | ||||
| B. caballi | 85.7 ± 2.1 | 8.6 | >17.5 | >17.5 | ||||
| T. equi | 78 ± 2.1 | 9.5 | >19.2 | >19.2 | ||||
a Half-maximal inhibitory concentration of extracts on piroplasm parasites in vitro. b Half-maximal effective concentration of extracts on the tested cell lines. The dose-response curve using nonlinear regression (curve fit analysis) was used to detect all of these values. The values obtained from the means of triplicate experiments. c Selective index calculated as the ratio of the EC50 of cell lines to the IC50 of each parasite.