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. 2020 Jan 25;21(3):787. doi: 10.3390/ijms21030787

Figure 1.

Figure 1

Figure 1

WIN 55-212.2 mesylate impacts on prostate cancer cell survival without affecting healthy prostate epithelial cells (a) Normal prostate epithelial cells (PNT-1), androgen-sensitive (LNCaP), and insensitive (PC-3 and DU-145) prostate cancer cells were incubated with increasing concentrations of WIN 55-22.2 mesylate and CBD for 24 h. Cell survival measurements by means of data obtained by crystal violet staining are shown (n = 3). p-Values were determined by Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001 WIN versus control (Untr); # p < 0.05, ### p < 0.001 CBD versus Untr. (b) Representative immunoblot of CB1, CB2 and TRPV1 expressions in PNT-1 and prostate cancer cell lines (c) Quantitative results of western blot analysis. Relative expressions were normalized respect to GAPDH. Each dot represents a biological replicate. Data express means ± SEMs. p-values were calculated by one-way ANOVA followed by Bonferroni’s multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001 versus PNT-1; # p < 0.05, ## p < 0.01 versus LNCaP; § p < 0.05 versus PC-3.