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. 2020 Jan 25;21(3):787. doi: 10.3390/ijms21030787

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The endocannabinoids affect the migration abilities of androgen-insensitive prostate cancer cells and CAFs’ reactivity by autocrine self-sustaining loops. (a,b) 3 × 10⁴ PC-3 (left) or DU-145 (right) cells were pre-treated for 24 h with different concentrations of AM281 and JTE-907 administrated alone or in combination. Tumor cells were allowed to migrate for 16 h towards complete medium (10% FBS). Cells maintained in serum-deprived medium were used as controls (Untr). Data represent means ± SEMs of three independent biological replicates. *** p < 0.001 versus Untr. (c) Representative immunoblot of α-SMA expression on CAFs treated with different concentrations of CBs antagonists, administrated alone or in combination, for 24 h.

The endocannabinoids affect the migration abilities of androgen-insensitive prostate cancer cells and CAFs’ reactivity by autocrine self-sustaining loops. (a,b) 3 × 10⁴ PC-3 (left) or DU-145 (right) cells were pre-treated for 24 h with different concentrations of AM281 and JTE-907 administrated alone or in combination. Tumor cells were allowed to migrate for 16 h towards complete medium (10% FBS). Cells maintained in serum-deprived medium were used as controls (Untr). Data represent means ± SEMs of three independent biological replicates. *** p < 0.001 versus Untr. (c) Representative immunoblot of α-SMA expression on CAFs treated with different concentrations of CBs antagonists, administrated alone or in combination, for 24 h.